Dr. Sachdev Sidhu

Dr. Sachdev Sidhu

Development of a toolkit of variants of the small protein ubiquitin that modulate function of a core regulator of cellular biology that is misrelated in numerous diseases (Molecular Cell 2016, 62:121-36). Team led by Dr. Sachdev Sidhu built upon the powerful high throughput synthetic antibody generation pipeline of the TRAC (Toronto Recombinant Antibody Centre), using phage-displayed libraries of small protein variants to generate biologically active modulators of protein function. Postdoctoral fellow Wei Zhang led the effort to characterize ubiquitin variants targeting HECT E3 ubiquitin ligases, which act as both inhibitors and activators of protein function. They established a general strategy for the highly efficient, systematic development of modulators targeting families of signaling proteins.