The course provides an overview and fundamental knowledge of recent optical microscopic techniques and their applications for biological research. The course consists of six different modules involving short lectures on specific imaging techniques by instructors, followed by presentations on research papers that highlight the application of these techniques by students. By the end of the course, each student will present a short research proposal on a research project of their interest, utilizing one of the imaging techniques covered by the course.

Week 1:

Part 1: Overview of the course; assigning papers for presentation; selecting tentative research topics for final proposal;

Part 2: Basics in flourescence microscopy (wide field, confocal and 2-photon microscopy) and imaging analysis

Week 2: FRET and FLIM

Week 3: Genetic (voltage/calcium/other) sensors

Week 4: Optogenetics

Week 5:

Part 1: Super-resolution

Part 2: Tracking microscopy

Week 6: Research proposal presentation by students

Coordinator: Dr. K. Okamoto & Dr. M. Zhen
Dates:  April - May, 2019
Place: TBA
Enrollment: Limited to 15 students